Ultrastructure of the endolymphatic sac in two-phase endolymphatic hydrops in the guinea pig

J Verheul*, JM Segenhout, EA Dunnebier, FWJ Albers, EH Blaauw, HP Wit

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    2 Citations (Scopus)

    Abstract

    Two-phase endolymphatic hydrops is a subtle experimental model for Meniere's disease. Chronic dysfunction of the endolymphatic sac, induced by dissection of the most distal part without causing damage to the intermediate part, is combined with increased endolymph production induced by administration of aldosterone which stimulates the Na/K-ATPase in the stria vascularis. A transmission electron microscopic study was performed on the endolymphatic sacs of four groups of guinea pig cochleas: controls; non-operated aldosterone-treated cochleas: operated (dissection of the endolymphatic sac) cochleas; operated and aldosterone-treated cochleas. Light and electron microscopy showed a normal morphology in the controls. Aldosterone treatment had no visible effect. Dissected ears revealed severe deviations. The epithelium of the intermediate sac was low, showed dilated lateral intercellular spaces indicating elevated fluid transport and displayed serious degenerative processes. Distally, the endolymphatic sac was completely blocked by newly formed bone. Additional aldosterone treatment had no cumulative effect on the dissected ears.

    Original languageEnglish
    Pages (from-to)345-350
    Number of pages6
    JournalActa oto-Laryngologica
    Volume119
    Issue number3
    Publication statusPublished - 1999

    Keywords

    • ultrastructure
    • endolymphatic sac
    • hydrops
    • guinea pig
    • INNER-EAR
    • MODEL

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