Unique genome replication mechanism of the archaeal virus AFV1

Mary Pina; Tamara Basta; Tessa Quax; Alexandra Joubert; Sonia Baconnais; Diego Cortez; Sarah Lambert; Eric Le Cam; Stephen D. Bell; Patrick Forterre; David Prangishvili

doi:10.1111/mmi.12630

Unique genome replication mechanism of the archaeal virus AFV1

Mary Pina, Tamara Basta, Tessa Quax, Alexandra Joubert, Sonia Baconnais, Diego Cortez, Sarah Lambert, Eric Le Cam, Stephen D. Bell, Patrick Forterre, David Prangishvili^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

16 Citations (Scopus)

Abstract

The exceptional genomic content and genome organi-zation of the Acidianus ﬁlamentous virus 1 (AFV1) thatinfects the hyperthermophilic archaeon Acidianushospitalis suggest that this virus might exploit anunusual mechanism of genome replication. An analy-sis of replicative intermediates of the viral genome bytwo-dimensional (2D) agarose gel electrophoresisrevealed that viral genome replication starts by theformation of a D-loop and proceeds via strand dis-placement replication. Characterization of replicativeintermediates using dark-ﬁeld electron microscopy, incombination with the 2D agarose gel electrophoresisdata, suggests that recombination plays a key role inthe termination of AFV1 genome replication throughthe formation of terminal loops. A terminal protein wasfound to be attached to the ends of the viral genome

Original language	English
Pages (from-to)	1313-1325
Number of pages	13
Journal	Molecular Microbiology
Volume	92
Issue number	6
DOIs	https://doi.org/10.1111/mmi.12630
Publication status	Published - Jun-2014
Externally published	Yes

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Unique genome replication mechanism of the archaeal virus AFV1
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title = "Unique genome replication mechanism of the archaeal virus AFV1",

abstract = "The exceptional genomic content and genome organi-zation of the Acidianus ﬁlamentous virus 1 (AFV1) thatinfects the hyperthermophilic archaeon Acidianushospitalis suggest that this virus might exploit anunusual mechanism of genome replication. An analy-sis of replicative intermediates of the viral genome bytwo-dimensional (2D) agarose gel electrophoresisrevealed that viral genome replication starts by theformation of a D-loop and proceeds via strand dis-placement replication. Characterization of replicativeintermediates using dark-ﬁeld electron microscopy, incombination with the 2D agarose gel electrophoresisdata, suggests that recombination plays a key role inthe termination of AFV1 genome replication throughthe formation of terminal loops. A terminal protein wasfound to be attached to the ends of the viral genome",

author = "Mary Pina and Tamara Basta and Tessa Quax and Alexandra Joubert and Sonia Baconnais and Diego Cortez and Sarah Lambert and {Le Cam}, Eric and Bell, {Stephen D.} and Patrick Forterre and David Prangishvili",

year = "2014",

month = jun,

doi = "10.1111/mmi.12630",

language = "English",

volume = "92",

pages = "1313--1325",

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T1 - Unique genome replication mechanism of the archaeal virus AFV1

AU - Pina, Mary

AU - Basta, Tamara

AU - Quax, Tessa

AU - Joubert, Alexandra

AU - Baconnais, Sonia

AU - Cortez, Diego

AU - Lambert, Sarah

AU - Le Cam, Eric

AU - Bell, Stephen D.

AU - Forterre, Patrick

AU - Prangishvili, David

PY - 2014/6

Y1 - 2014/6

N2 - The exceptional genomic content and genome organi-zation of the Acidianus ﬁlamentous virus 1 (AFV1) thatinfects the hyperthermophilic archaeon Acidianushospitalis suggest that this virus might exploit anunusual mechanism of genome replication. An analy-sis of replicative intermediates of the viral genome bytwo-dimensional (2D) agarose gel electrophoresisrevealed that viral genome replication starts by theformation of a D-loop and proceeds via strand dis-placement replication. Characterization of replicativeintermediates using dark-ﬁeld electron microscopy, incombination with the 2D agarose gel electrophoresisdata, suggests that recombination plays a key role inthe termination of AFV1 genome replication throughthe formation of terminal loops. A terminal protein wasfound to be attached to the ends of the viral genome

AB - The exceptional genomic content and genome organi-zation of the Acidianus ﬁlamentous virus 1 (AFV1) thatinfects the hyperthermophilic archaeon Acidianushospitalis suggest that this virus might exploit anunusual mechanism of genome replication. An analy-sis of replicative intermediates of the viral genome bytwo-dimensional (2D) agarose gel electrophoresisrevealed that viral genome replication starts by theformation of a D-loop and proceeds via strand dis-placement replication. Characterization of replicativeintermediates using dark-ﬁeld electron microscopy, incombination with the 2D agarose gel electrophoresisdata, suggests that recombination plays a key role inthe termination of AFV1 genome replication throughthe formation of terminal loops. A terminal protein wasfound to be attached to the ends of the viral genome

U2 - 10.1111/mmi.12630

DO - 10.1111/mmi.12630

M3 - Article

C2 - 24779456

SN - 0950-382X

VL - 92

SP - 1313

EP - 1325

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 6

ER -

Unique genome replication mechanism of the archaeal virus AFV1

Abstract

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