Unraveling structure and dynamics by confocal microscopy: From starch to organic semiconductors

Marianna Manca

Research output: ThesisThesis fully internal (DIV)

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Confocal Laser Scanning Microscopy (CLSM) is a powerful tool to correlate morphology and spatial resolved spectroscopy due to the high spatial resolution achievable. The possibility to investigate also living tissues makes CLSM a popular technique in biology and less conventional fields.
In this thesis the properties and structure of two different systems have been investigated: amylose-lipid inclusion complex in starch granules and organic bulk -heterojunctions.
The tendency of amylose to form inclusion complexes with guest molecules has been an object of wide interest due to its fundamental role in food processing.
Here we investigated the interactions between amylose contained in starch granules from different botanical sources and fluorescent lipophilic molecules below gelatinization temperature.
By combining CLSM and spatial resolved photoluminescence spectroscopy, we were able to discriminate the presence of amylose in the peripheral region of starch granules associating a clear optical fingerprint to the amylose-lipid interactions. By performing real time CLSM measurements, we were able to investigate the dynamics of these interactions, estimating within tens of seconds the time necessary for this process to occur.
Charge transfer state (CTS) emission in organic bulk heterojunctions has been demonstrated to be an important loss mechanism: a better understanding of this recombination process is fundamental for the improvement of organic solar cells.
The relation between photophysical and morphological features of a prototypical organic bulk heterojunction are investigated in blends with different donor acceptor ratios, assigning unambiguously the CTS emission to microscopical regions in which the intermixing of the two organic semiconductors is higher.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
  • Loi, Maria, Supervisor
Award date22-May-2015
Place of Publication[Groningen]
Print ISBNs978-90-367-7883-1
Electronic ISBNs978-90-367-7882-4
Publication statusPublished - 2015

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