Use of gated N-13-NH3 micro-PET to examine left ventricular function in rats

Mariusz K. Szymanski, Silvana Kruizinga, Rene A. Tio, Antoon T. M. Willemsen, Michael A. Schaefers, Lars Stegger, Rudi A. Dierckx, Hans L. Hillege, Riemer H. J. A. Slart*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Introduction: Myocardial perfusion gating techniques offer the possibility of measurement of left ventricular end-systolic (ESV) and end-diastolic volume (EDV) and left ventricular ejection fraction (LVEF) in clinical and preclinical trials. The aim of this study was to evaluate left ventricular volumes (LVV) and LVEF with N-13-NH3 in comparison with the reference F-18-FDG in different rat models.

Methods: In this study, 18 male Wistar rats, 12 control rats and 6 rats with myocardial infarction (MI) were imaged with micro-PET. The rats were scanned with gated N-13-NH3 and F-18-FDG sequentially for the assessment of LVV and LVEF. A validated three-dimensional segmentation algorithm was used to calculate LVV and LVEF.

Results: Mean LVEF measured with N-13-NH3 was 45.6+/-8.9 and 75.3+/-9.4%, mean ESV was 0.40+/-0.12 and 0.14+/-0.11 ml, and mean EDV was 0.53+/-16 and 0.75+/-0.18 ml for MI and control rats, respectively. Moderate to good correlations were observed between values of N-13-NH3 and F-18-FDG for calculation of ESV [r=0.80, P

Conclusion: Correlations were moderate to good for the assessment of ESV, EDV and LVEF between gated N-13-NH3 and F-18-FDG. LVEF was underestimated with gated N-13-NH3 in rats with myocardial infarction. in healthy rats, LV volumes and LVEF can be measured reproducibly with either approach. (C) 2012 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)724-729
Number of pages6
JournalNuclear Medicine and Biology
Volume39
Issue number5
DOIs
Publication statusPublished - Jul-2012

Keywords

  • N-13-NH3 micro-PET
  • Cardiac function
  • IDIOPATHIC DILATED CARDIOMYOPATHY
  • CORONARY-ARTERY-DISEASE
  • MYOCARDIAL BLOOD-FLOW
  • SMALL-ANIMAL PET
  • EJECTION FRACTION
  • F-18-FDG PET
  • FDG-PET
  • MRI
  • VALIDATION
  • VOLUMES

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