Utilization of Glycosyltransferases for the Synthesis of a Densely Packed Hyperbranched Polysaccharide Brush Coating as Artificial Glycocalyx

Jeroen van der Vlist, Iris Schonen, Katja Loos*, Iris Schönen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)
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Abstract

Densely packed polysaccharide brushes consisting of alpha-D-glucose residues were grafted from modified silicon substrates. Potato phosphorylase was herein used to grow linear polysaccharide chains from silicon tethered maltoheptaose oligosaccharides using glucose-1-phosphate as donor substrate. The combined use of potato phosphorylase and Deinococcus geothermalis branching enzyme resulted in a hyperbranched brush coating as the latter one redistributes short oligosaccharides from the alpha(1-4)-linked position to the alpha(1-6)-linked position in the polysaccharide brush. The obtained grafting density of the brushes was estimated on 1.89 nm(-2) while the thickness was measured with ellipsometric techniques and, determined to be between 12.2 and 20.2 nm.

Original languageEnglish
Pages (from-to)3728-3732
Number of pages5
JournalBiomacromolecules
Volume12
Issue number10
DOIs
Publication statusPublished - Oct-2011

Keywords

  • POLY(ETHYLENE OXIDE) BRUSHES
  • SELECTIVE REDUCING AGENT
  • ENDOTHELIAL GLYCOCALYX
  • THERMAL-DEGRADATION
  • SURFACTANT POLYMERS
  • PROTEIN ADSORPTION
  • PLATELET-ADHESION
  • COPOLYMERS
  • PREVENTION
  • HEPARIN

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