Virosomes as a vaccine delivery system

L Bungener*, A Huckriede, J Wilschut, T Daemen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Virosomes retain the cell entry, and membrane fusion characteristics of the native virus. Thus, influenza-derived virosomes are taken up by cells via receptor-mediated endocytosis, which directs the particles to the endosomes. Subsequently, the virosomal membrane fuses with the endosomal membrane from within the lumen of the endosomes. The fusion process establishes continuity between the lumen of the virosome, and the cell cytosol. Therefore, protein encapsulated in virosomes will be delivered into the cytosol of the cell as shown here for the A subunit of diphtheria toxin. Antigen-containing virosomes interacting with APCs are expected to introduce part of the antigen into the cell cytosol and, thus, into the MHC class I presentation pathway. Delivery of antigens in vivo indeed results in efficient priming of a class I MHC-restricted CTL response making virosomes a promising vehicle for in vivo targeting.

Original languageEnglish
Title of host publicationEFIS 2000: 14TH EUROPEAN IMMUNOLOGY MEETING
EditorsA Mackiewicz, M Kurpise, J Zeromski
Place of Publication40128 BOLOGNA
PublisherMEDIMOND PUBLISHING CO
Pages845-850
Number of pages6
ISBN (Print)*************
Publication statusPublished - 2000
Event14th European Immunology Meeting (EFIS 2000) - , Poland
Duration: 23-Sep-200027-Sep-2000

Other

Other14th European Immunology Meeting (EFIS 2000)
Country/TerritoryPoland
Period23/09/200027/09/2000

Keywords

  • INFLUENZA-VIRUS ENVELOPES
  • FUSION

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