TY - JOUR
T1 - Virulence and antimicrobial resistance of Staphylococcus aureus isolated from bloodstream infections and pneumonia in Southern Poland
AU - Pomorska-Wesolowska, Monika
AU - Chmielarczyk, Agnieszka
AU - Chlebowicz, Monika
AU - Ziolkowski, Grzegorz
AU - Szczypta, Anna
AU - Natkaniec, Joanna
AU - Romaniszyn, Dorota
AU - Pobiega, Monika
AU - Dzikowska, Miroslawa
AU - Krawczyk, Lech
AU - Koziol, Joanna
AU - Wojkowska-Mach, Jadwiga
PY - 2017/12
Y1 - 2017/12
N2 - Objectives: Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities. The aim of this study was to analyse the resistance, virulence, and epidemiological and genetic relationships of S. aureus from bloodstream infections (BSIs) and pneumonia from patients in Southern Poland.Methods: All strains were tested for antimicrobial susceptibility using the disk diffusion method. Etest was also performed for vancomycin, teicoplanin, tigecycline, oxacillin, cefoxitin and penicillin. PCR amplification was used to detect selected virulence genes. The genetic similarity of methicillin-resistant S. aureus (MRSA) isolates was determined by spa typing and pulsed-field gel electrophoresis (PFGE). Using the BURP algorithm and the Ridom SpaServer database, spa types were clustered into different clonal complexes (spa-CCs).Results and conclusions: MRSA strains were observed at a prevalence of 26.7%, but 88.6% of hospital-acquired infections were MRSA, with no difference between BSIs and pneumonia. The highest resistance was observed to erythromycin and tobramycin. None of the strains were resistant to linezolid, glycopeptides or tigecycline. The strains had no significant virulence factors and the number of virulence genes present did not correlate with the degree of drug resistance. PFGE typing showed relatively high diversity of strains. The majority of isolates belonged to spa type t003 (CC5).
AB - Objectives: Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities. The aim of this study was to analyse the resistance, virulence, and epidemiological and genetic relationships of S. aureus from bloodstream infections (BSIs) and pneumonia from patients in Southern Poland.Methods: All strains were tested for antimicrobial susceptibility using the disk diffusion method. Etest was also performed for vancomycin, teicoplanin, tigecycline, oxacillin, cefoxitin and penicillin. PCR amplification was used to detect selected virulence genes. The genetic similarity of methicillin-resistant S. aureus (MRSA) isolates was determined by spa typing and pulsed-field gel electrophoresis (PFGE). Using the BURP algorithm and the Ridom SpaServer database, spa types were clustered into different clonal complexes (spa-CCs).Results and conclusions: MRSA strains were observed at a prevalence of 26.7%, but 88.6% of hospital-acquired infections were MRSA, with no difference between BSIs and pneumonia. The highest resistance was observed to erythromycin and tobramycin. None of the strains were resistant to linezolid, glycopeptides or tigecycline. The strains had no significant virulence factors and the number of virulence genes present did not correlate with the degree of drug resistance. PFGE typing showed relatively high diversity of strains. The majority of isolates belonged to spa type t003 (CC5).
KW - Virulence
KW - Resistance
KW - Bloodstream infection
KW - Pneumonia
KW - Typing
KW - METHICILLIN-RESISTANT
U2 - 10.1016/j.jgar.2017.07.009
DO - 10.1016/j.jgar.2017.07.009
M3 - Article
SN - 2213-7165
VL - 11
SP - 100
EP - 104
JO - Journal of Global Antimicrobial Resistance
JF - Journal of Global Antimicrobial Resistance
ER -