Viscoelasticity of biofilms and their recalcitrance to mechanical and chemical challenges

Brandon W. Peterson, Yan He, Yijin Ren, Aidan Zerdoum, Matthew R. Libera, Prashant K. Sharma, Arie-Jan van Winkelhoff, Danielle Neut, Paul Stoodley, Henny C. van der Mei*, Henk J. Busscher

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

144 Citations (Scopus)
417 Downloads (Pure)

Abstract

We summarize different studies describing mechanisms through which bacteria in a biofilm mode of growth resist mechanical and chemical challenges. Acknowledging previous microscopic work describing voids and channels in biofilms that govern a biofilms response to such challenges, we advocate a more quantitative approach that builds on the relation between structure and composition of materials with their viscoelastic properties. Biofilms possess features of both viscoelastic solids and liquids, like skin or blood, and stress relaxation of biofilms has been found to be a corollary of their structure and composition, including the EPS matrix and bacterial interactions. Review of the literature on viscoelastic properties of biofilms in ancient and modern environments as well as of infectious biofilms reveals that the viscoelastic properties of a biofilm relate with antimicrobial penetration in a biofilm. In addition, also the removal of biofilm from surfaces appears governed by the viscoelasticity of a biofilm. Herewith, it is established that the viscoelasticity of biofilms, as a corollary of structure and composition, performs a role in their protection against mechanical and chemical challenges. Pathways are discussed to make biofilms more susceptible to antimicrobials by intervening with their viscoelasticity, as a quantifiable expression of their structure and composition.

Original languageEnglish
Pages (from-to)234-245
Number of pages12
JournalFEMS Microbiology Reviews
Volume39
Issue number2
Early online date3-Feb-2015
DOIs
Publication statusPublished - Mar-2015

Keywords

  • biofilm
  • structure
  • extracellular polymeric substances (EPS)
  • antimicrobial penetration
  • detachment
  • viscoelasticity
  • EXTRACELLULAR POLYMERIC SUBSTANCES
  • TRANSMISSION ELECTRON-MICROSCOPY
  • GRAM-NEGATIVE BACTERIA
  • TRACHEAL TUBE BIOFILM
  • PSEUDOMONAS-AERUGINOSA
  • ESCHERICHIA-COLI
  • MICROBIAL BIOFILMS
  • ORAL BIOFILM
  • FLUID SHEAR
  • SHOCK-WAVES

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