Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy

Chris van Ewijk, Sourav Maity, Wouter H Roos*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Dynamic processes and structural changes of biological molecules are essential to life. While conventional atomic force microscopy (AFM) is able to visualize molecules and supramolecular assemblies at sub-nanometer resolution, it cannot capture dynamics because of its low imaging rate. The introduction of high-speed atomic force microscopy (HS-AFM) solved this problem by providing a large increase in imaging velocity. Using HS-AFM, one is able to visualize dynamic molecular events with high spatiotemporal resolution under near-to physiological conditions. This approach opened new windows as finally dynamics of biomolecules at sub-nanometer resolution could be studied. Here we describe the working principles and an operation protocol for HS-AFM imaging and characterization of biological samples in liquid.

Original languageEnglish
Title of host publicationSingle Molecule Analysis
EditorsI. Heller, D. Dulin, E.J. Peterman
PublisherHumana, New York, NY
Pages355-372
Number of pages18
DOIs
Publication statusPublished - 2024

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana, New York, NY
Volume2694
ISSN (Print)1064-3745

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