Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy

Chris van Ewijk, Sourav Maity, Wouter H Roos*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Dynamic processes and structural changes of biological molecules are essential to life. While conventional atomic force microscopy (AFM) is able to visualize molecules and supramolecular assemblies at sub-nanometer resolution, it cannot capture dynamics because of its low imaging rate. The introduction of high-speed atomic force microscopy (HS-AFM) solved this problem by providing a large increase in imaging velocity. Using HS-AFM, one is able to visualize dynamic molecular events with high spatiotemporal resolution under near-to physiological conditions. This approach opened new windows as finally dynamics of biomolecules at sub-nanometer resolution could be studied. Here we describe the working principles and an operation protocol for HS-AFM imaging and characterization of biological samples in liquid.

Original languageEnglish
Title of host publicationSingle Molecule Analysis
EditorsI. Heller, D. Dulin, E.J. Peterman
PublisherHumana, New York, NY
Number of pages18
Publication statusPublished - 2024

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana, New York, NY
ISSN (Print)1064-3745

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