Dataset: Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice

  • Katie Amssoms (Creator)
  • Philip Born (Creator)
  • Max Beugeling (Creator)
  • Ben De Clerck (Creator)
  • Ellen Van Gulck (Creator)
  • Wouter Hinrichs (Creator)
  • Erik Frijlink (Creator)
  • Niels Grasmeijer (Creator)
  • Guenter Kraus (Creator)
  • Roger Sutmuller (Creator)
  • Kenny Simmen (Creator)
  • Lieven Baert (Creator)

Dataset

Description

Vaccination is one of the most effective and efficient ways to control the spread of more than thirty infectious diseases.
In this study we investigated whether a core-shell based implant, containing ovalbumin as core material and poly(DL-lactic-co-glycolic acid) of various monomer ratios as shell material can be used to obtain such a booster release. An in vitro release study showed that the lag time after which the ovalbumin was released from the core-shell implant increased with increasing lactic to glycolic acid ratio of the polymer and ranged from 3–6 weeks. Fluorescence spectroscopy showed minimal differences between native ovalbumin and ovalbumin from core-shell implants that were incubated until just before the observed in vitro release. In addition, mice immunized with a subcutaneous inserted core-shell implant containing ovalbumin showed an ovalbumin-specific IgG1 antibody response after a lag time of 4 or 6–8 weeks.
Datum van beschikbaarheid17-aug-2018
UitgeverUniversity of Groningen

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