The locomotor performance of Drosophila is automatically determined in a temperature-controlled arena that produces fast and accurate temperature changes in time and space. The temperature-controlled arena is related to the following scripts: TemperaturePhases.m TemperaturePhases_ShiftingComfortableTIle.m FlySteps_Analysis.r FlySteps_Analysis.m Fly_Tracker Examples For further questions on set-up and usage contact the authors.