The Eastern Mediterranean (EM) is one of the most impoverished marine reserves of our planet. Although this condition is generally attributed to post-Suez anthropogenic impact, it is unlikely that the coastal waters of the EM were in a pristine state before recent industrialization. The EM is arguably the oldest continuously and intensively humanized seascape of the world. From Homeric Troy to Biblical Ashkelon, the basin is surrounded by some of the earliest cities in history. Their ruins are abundant with fishing equipment, mollusc shells, and fish bones; all tangible evidence for millennia of human-fisheries interaction. What was the nature and scale of this interaction? How large was anthropogenic impact on fisheries? This project aims to explore these questions through the extraction of catch composition and size range data from a rare fish bone assemblage from the ancient harbour of Kinet Höyük (5000 and 700 BP), located near the Syrian border of Turkey. This study is the first of its kind to be conducted in the region and the results will constitute a reproducible baseline for future conservation research in the EM.