A fast and inexpensive DNA extraction/purification protocol for brown macroalgae

Galice Hoarau*, James A. Coyer, Wytze T. Stam, Jeanine L. Olsen

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

49 Citaten (Scopus)

Samenvatting

Here we describe a rapid method for extracting DNA from dried brown algae material using a microtitre plate system in conjunction with a milling instrument. The method allows the preparation of nuclear and organelle DNA of quality suitable for polymerase chain reaction amplification. It combines high throughput with low cost per sample: DNA from 192 samples can be extracted in c. 3 h for <EURO0.40 per sample, nearly tenfold cheaper than commercially available kits. Furthermore, by using microtitre plates, efficient storage and downstream processing is facilitated.

Originele taal-2English
Pagina's (van-tot)191-193
Aantal pagina's3
TijdschriftMolecular Ecology Notes
Volume7
Nummer van het tijdschrift2
DOI's
StatusPublished - mrt-2007

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