A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids

Claudia Goehner, Maja Weber, Dionne S. Tannetta, Tanja Groten, Torsten Ploesch, Marijke M. Faas, Sicco A. Scherjon, Ekkehard Schleussner, Udo R. Markert, Justine S. Fitzgerald*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

15 Citaten (Scopus)


ProblemThe pregnancy-associated disease preeclampsia is related to the release of syncytiotrophoblast extracellular vesicles (STBEV) by the placenta. To improve functional research on STBEV, reliable and specific methods are needed to quantify them. However, only a few quantification methods are available and accepted, though imperfect. For this purpose, we aimed to provide an enzyme-linked sorbent assay (ELSA) to quantify STBEV in fluid samples based on their microvesicle characteristics and placental origin.

Method of StudyEx vivo placenta perfusion provided standards and samples for the STBEV quantification. STBEV were captured by binding of extracellular phosphatidylserine to immobilized annexin V. The membranous human placental alkaline phosphatase on the STBEV surface catalyzed a colorimetric detection reaction.

Results and ConclusionThe described ELSA is a rapid and simple method to quantify STBEV in diverse liquid samples, such as blood or perfusion suspension. The reliability of the ELSA was proven by comparison with nanoparticle tracking analysis.

Originele taal-2English
Pagina's (van-tot)582-588
Aantal pagina's7
TijdschriftAmerican Journal of Reproductive Immunology
Nummer van het tijdschrift6
StatusPublished - jun-2015

Citeer dit