A peptide hydroxamate library for enrichment of metalloproteinases: towards an affinity-based metalloproteinase profiling protocol

Paul Geurink, Theo Klein, Michiel Leeuwenburgh, Gijs van der Marel, Henk Kauffman, Rainer Bischoff*, Herman Overkleeft

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

13 Citaten (Scopus)

Samenvatting

A compound library of 96 enantiopure N-terminal succinyl hydroxamate functionalized peptides was synthesized on solid phase. All compounds were tested for their inhibitory potential towards MMP-9, MMP-12 and ADAM-17, which led to the identification of both broad spectrum inhibitors and metalloproteinase-selective ones. Eight potent and less potent inhibitors were immobilized on Sepharose beads and evaluated in solid-phase enrichment of active MMP-9, MMP-12 and ADAM-17. In addition, one of these inhibitors was used for solid-phase enrichment of endogenous ADAM-17 from a complex proteome ( a lysate prepared from cultured A549 cells).

Originele taal-2English
Pagina's (van-tot)1244-1250
Aantal pagina's7
TijdschriftOrganic and Biomolecular Chemistry
Volume6
Nummer van het tijdschrift7
DOI's
StatusPublished - 22-feb.-2008

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