A sensor for quantification of macromolecular crowding in living cells

Arnold J. Boersma*, Inge S. Zuhorn, Bert Poolman

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

122 Citaten (Scopus)

Samenvatting

Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.

Originele taal-2English
Pagina's (van-tot)227-229
Aantal pagina's3
TijdschriftNature Methods
Volume12
Nummer van het tijdschrift3
DOI's
StatusPublished - mrt-2015

Citeer dit