A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules

Melany Rios-Morales, Mara P. H. van Trijp, Christiane Rosch, Ran An, Theo Boer, Albert Gerding, Naomi de Ruiter, Martijn Koehorst, M. Rebecca Heiner-Fokkema, Henk A. Schols, Dirk-Jan Reijngoud, Guido J. E. J. Hooiveld, Barbara M. Bakker*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

2 Citaten (Scopus)
21 Downloads (Pure)

Samenvatting

Detailed knowledge on the fate of dietary components inside the human intestinal tract is lacking. Access to this inner world of digestion is now possible through novel human gastrointestinal sampling capsules. Due to the novelty of such devices, no methodology has been published to stabilise and analyse the resulting samples. A complicating factor is that excretion of such capsules in faeces may take days, while degradation of the dietary components continues. Therefore a stabilising reagent should be pre-loaded in the capsule to ensure the measurement of a representative sample. Considering the small volume of recovered samples, analytical methods must be optimized to collect as many data as possible from little material. We present a complete workflow for stabilising and analysing the fermentation status of dietary fibres in such samples, including microbiota, fibre degradation, and short chain fatty acids. The final quenching reagent was designed based on safety and effectiveness to inhibit fructo- and galacto-oligosaccharides degradation and short chain fatty acids production by human ileostomy microbiota, and subsequently validated in faecal samples. The final composition of the stock quenching reagent is 175 mM Tris, 525 mM NaCl, 35 mM EDTA, 12% SDS, and 8 M urea at pH 8.5.

Originele taal-2English
Artikelnummer8133
Aantal pagina's14
TijdschriftScientific Reports
Volume11
Nummer van het tijdschrift1
DOI's
StatusPublished - 14-apr.-2021

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