TY - JOUR
T1 - A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast
AU - Novarina, Daniele
AU - Koutsoumpa, Andriana
AU - Milias-Argeitis, Andreas
N1 - © 2022 The Author(s).
PY - 2022/6/17
Y1 - 2022/6/17
N2 - CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.org/kits/moclo-ytk/). This step-by-step protocol guides the reader from sgRNA design to verification of the desired genome editing event and provides preassembled plasmids for cloning the sgRNA(s), making this technology easily accessible to any yeast research group. For complete details on the use and execution of this protocol, please refer to Novarina et al. (2021).
AB - CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.org/kits/moclo-ytk/). This step-by-step protocol guides the reader from sgRNA design to verification of the desired genome editing event and provides preassembled plasmids for cloning the sgRNA(s), making this technology easily accessible to any yeast research group. For complete details on the use and execution of this protocol, please refer to Novarina et al. (2021).
KW - CRISPR-Cas Systems/genetics
KW - Gene Editing/methods
KW - Plasmids/genetics
KW - Saccharomyces cerevisiae/genetics
KW - Saccharomycetales/genetics
U2 - 10.1016/j.xpro.2022.101358
DO - 10.1016/j.xpro.2022.101358
M3 - Article
C2 - 35712010
SN - 2666-1667
VL - 3
JO - STAR protocols
JF - STAR protocols
IS - 2
M1 - 101358
ER -