Adaptative transcriptional response of Dietzia cinnamea P4 strain to sunlight simulator

Luciano Procopio*, Marcelo Padula, Jan Dirk van Elsas, Lucy Seldin

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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Responses to sunlight exposure of the oil-degrading Dietzia cinnamea P4 strain were evaluated by transcriptional levels of SOS genes, photoreactivation and genes involved in tolerance to high levels of reactive oxygen species. The P4 strain was exposed for 1 and 2 h and the magnitude of level changes in the mRNA was evaluated by qPCR. The results described the activation of the SOS system, with the decline of the repressor lexA gene levels and the concomitant increase of recA and uvrAD genes levels. The genes that participate in the photoreactivation process were also responsive to sunlight. The phrB gene encoding deoxyribodipyrimidine photo-lyase had its expression increased after 1-h exposure, while the phytAB genes showed a progressive increase over the studied period. The protective genes against reactive oxygen species, catalases, superoxides, peroxidases, and thioredoxins, had their expression rates detected under the conditions validated in this study. These results show a fast and coordinated response of genes from different DNA repair and tolerance mechanisms employed by strain P4, suggesting a complex concerted protective action against environmental stressors.

Originele taal-2English
Pagina's (van-tot)1701-1708
Aantal pagina's8
TijdschriftArchives of Microbiology
Volume202
Nummer van het tijdschrift7
Vroegere onlinedatum15-apr-2020
DOI's
StatusPublished - sep-2020

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