Altered immune response of immature dendritic cells upon dengue virus infection in the presence of specific antibodies

Silvia Torres , Jacky Flipse, Vinit C. Upasani, Heidi van der Ende-Metselaar, Silvio Urcuqui-Inchima, Jolanda M. Smit, Izabela A. Rodenhuis-Zybert*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

3 Citaten (Scopus)

Samenvatting

Dengue virus (DENV) replication is known to prevent maturation of infected dendritic cells (DCs) thereby impeding the development of adequate immunity. During secondary DENV infection, dengue-specific antibodies can suppress DENV replication in immature DCs (immDCs), however how dengue-antibody complexes (DENV-IC) influence the phenotype of DCs remains elusive. Here, we evaluated the maturation state and cytokine profile of immDCs exposed to DENV-ICs. Indeed, DENV infection of immDCs in the absence of antibodies was hallmarked by blunted upregulation of CD83, CD86 and the major histocompatibility complex molecule HLA-DR. In contrast, DENV infection in the presence of neutralizing antibodies triggered full DC maturation and induced a balanced inflammatory cytokine response. Moreover, DENV infection under non-neutralizing conditions prompted upregulation of CD83 and CD86 but not HLA-DR, and triggered production of pro inflammatory cytokines. The effect of DENV-IC was found to be dependent on the engagement of FcyRIla. Altogether, our data show that the presence of DENV-IC alters the phenotype and cytokine profile of DCs.

Originele taal-2English
Pagina's (van-tot)1584-1591
Aantal pagina's8
TijdschriftThe Journal of general virology
Volume97
Nummer van het tijdschrift7
DOI's
StatusPublished - jul-2016

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