An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication

Mario Mauthe, Martijn Langereis, Jennifer Jung, Xingdong Zhou, Alex Jones, Wienand Omta, Sharon A. Tooze, Bjoern Stork, Soren Riis Paludan, Tero Ahola, Dave Egan, Christian Behrends, Michal Mokry, Cornelis de Haan, Frank van Kuppeveld, Fulvio Reggiori*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

34 Citaten (Scopus)
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Autophagy is a catabolic process regulated by the orchestrated action of the autophagy-related (ATG) proteins. Recent work indicates that some of the ATG proteins also have autophagy-independent roles. Using an unbiased siRNA screen approach, we explored the extent of these unconventional functions of ATG proteins. We determined the effects of the depletion of each ATG proteome component on the replication of six different viruses. Our screen reveals that up to 36% of the ATG proteins significantly alter the replication of at least one virus in an unconventional fashion. Detailed analysis of two candidates revealed an undocumented role for ATG13 and FIP200 in picornavirus replication that is independent of their function in autophagy as part of the ULK complex. The high numbers of unveiled ATG gene-specific and pathogen specific functions of the ATG proteins calls for caution in the interpretation of data, which rely solely on the depletion of a single ATG protein to specifically ablate autophagy.

Originele taal-2English
Pagina's (van-tot)619-635
Aantal pagina's17
TijdschriftThe Journal of Cell Biology
Volume214
Nummer van het tijdschrift5
DOI's
StatusPublished - 29-aug-2016

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