Analysis of protein dynamics during cytokinesis in budding yeast

S. Okada, C. Wloka, E. Bi*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

5 Citaten (Scopus)

Samenvatting

Cytokinesis is essential for development and survival of all organisms by increasing cell number and diversity. It is a highly regulated process that requires spatiotemporal coordination of hundreds of proteins functioning in the assembly, constriction, and disassembly of a contractile actomyosin ring, targeted vesicle fusion, and localized extracellular matrix remodeling. Cytokinesis has been studied in multiple systems with a wide range of technologies to learn the common principles. In this chapter, we describe the analysis of protein dynamics during cytokinesis in the budding yeast Saccharomyces cerevisiae by several live-cell imaging methods. This, in combination with the power of yeast genetics, has yielded novel insights into the mechanism of cytokinesis. Similar approaches are increasingly used to study this fundamental process in more complex systems.

Originele taal-2English
TitelCytokinesis
RedacteurenA Echard
UitgeverijElsevier
Hoofdstuk3
Pagina's25-45
Aantal pagina's21
Uitgave1
ISBN van elektronische versie978-0-12-809792-2
ISBN van geprinte versie978-0-12-809673-4
DOI's
StatusPublished - 2017

Publicatie series

NaamMethods in Cell Biology
UitgeverijElsevier
Volume137
ISSN van geprinte versie0091-679X

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