Analysis of repetitive DNA in chromosomes by flow cytometry

Julie Brind'Amour, Peter M. Lansdorp*

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: ArticleAcademicpeer review

19 Citaten (Scopus)

Samenvatting

We developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (FISH), called CFF, to analyze repetitive DNA in chromosomes using FISH with directly labeled peptide nucleic acid (PNA) probes. We used CFF to measure the abundance of interstitial telomeric sequences in Chinese hamster chromosomes and major satellite sequences in mouse chromosomes. Using CFF we also identified parental homologs of human chromosome 18 with different amounts of repetitive DNA.

Originele taal-2English
Pagina's (van-tot)484-U71
Aantal pagina's5
TijdschriftNature Methods
Volume8
Nummer van het tijdschrift6
DOI's
StatusPublished - jun-2011

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