Analysis of the Tumor Microenvironment Transcriptome via NanoString mRNA and miRNA Expression Profiling

Marie-Noël M'Boutchou, Léon C van Kempen

Onderzoeksoutput: ArticleAcademicpeer review

11 Citaten (Scopus)

Samenvatting

UNLABELLED: Gene expression analysis in the tumor microenvironment using archived clinical samples is challenging because of formalin fixation, RNA degradation, and limiting sample volume. NanoString gene expression profiling is a RNA-DNA hybrid capture technology that does not require PCR and can accurately quantify the expression of to 800 transcripts in a single reaction. The technology requires 50-100 ng of RNA, which can be degraded (

EDITOR: is this correct?) to a 200 bp fragment size. In contrast to amplification technologies, nanoString counts the actual numbers of transcripts that are captured with transcript-specific and fluorescently-barcoded probes. This chapter describes protocols for RNA extraction, quantification, mRNA and miRNA profiling and data analysis.

Originele taal-2English
Pagina's (van-tot)291-310
Aantal pagina's20
TijdschriftMethods in Molecular Biology
Volume1458
DOI's
StatusPublished - 2016
Extern gepubliceerdJa

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