Analysis of two additional signaling molecules in Streptomyces coelicolor and the development of a butyrolactone-specific reporter system

Nai-Hua Hsiao, Satoshi Nakayama, Maria Elena Merlo, Marcel de Vries, Robert Bunet, Shigeru Kitani, Takuya Nihira, Eriko Takano*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

51 Citaten (Scopus)
492 Downloads (Pure)


gamma-Butyrolactone bacterial hormones regulate antibiotic production and morphological differentiation in Streptomyces species. One gamma-butyrolactone, SCB1, has been previously characterized in Streptomyces coelicolor. Here we report the characterization of two additional gamma-butyrolactones, named SCB2 (2-[1'-hydroxyoctyl]-3-hydroxymethylbutanolide) and SCB3 (2-[1'-hydroxy-6'-methyloctyl]-3-hydroxymethylbutanolide), possessing an antibiotic stimulatory activity. To elucidate the specificity determinants of these ligands for the receptor protein, ScbR, 30 chemically synthesized gamma-butyrolactone analogs were tested by utilizing the release of ScbR from DNA upon binding to a gamma-butyrolactone, which can be detected by kanamycin resistance. The butyrolactone detection method developed here revealed that ScbR shows preference toward a ligand possessing a 7-10 carbon C-2 side chain, a C-1'-beta-hydroxyl group, and a C-6'-methyl branch that coincides with SCB3. Moreover, this method was successfully used to screen for potential gamma-butyrolactone producers from commercial-antibiotic-producing Streptomyces.

Originele taal-2English
Pagina's (van-tot)951-960
Aantal pagina's10
TijdschriftChemistry & Biology
Nummer van het tijdschrift9
StatusPublished - 25-sep.-2009

Citeer dit