Samenvatting
Antibody-free approaches for quantitative LC-MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature peptides by fractionated protein precipitation; enrichment of target proteins and signature peptides by reversed-phase and ion-exchange solid-phase extraction; and enrichment of target proteins and signature peptides by (antibody-free) affinity-solid-phase extraction.
Originele taal-2 | English |
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Pagina's (van-tot) | 763-779 |
Aantal pagina's | 17 |
Tijdschrift | Bioanalysis |
Volume | 7 |
Nummer van het tijdschrift | 6 |
DOI's | |
Status | Published - apr.-2015 |