@article{37b6e14d15b24bc6a5f1c095db2d8cfd,
title = "Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography",
abstract = "Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell-cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer.",
keywords = "Archaea, Electron cryotomography, S-layers, Subtomogram averaging, Sulfolobus",
author = "Lavinia Gambelli and Meyer, {Benjamin H.} and Mathew McLaren and Kelly Sanders and Quax, {Tessa E. F.} and Gold, {Vicki A.M.} and Albers, {Sonja Verena} and Bertram Daum",
note = "Funding Information: ACKNOWLEDGMENTS. We thank Werner K{\"u}hlbrandt and Deryck Mills [Max Planck Institute (MPI) of Biophysics in Germany] for allowing us to use the Polara and Jeol electron microscopes. This project was funded by the Max Funding Information: Planck Society (to B.D.), the University of Exeter Research Fellow{\textquoteright}s Startup grant (to B.D.), and the European Research Council (ERC) starting grant “MICROROBOTS” (to L.G., M.M., K.S., and B.D.). Funding Information: We thank Werner K?hlbrandt and Deryck Mills [Max Planck Institute (MPI) of Biophysics in Germany] for allowing us to use the Polara and Jeol electron microscopes. This project was funded by the Max Planck Society (to B.D.), the University of Exeter Research Fellow's Startup grant (to B.D.), and the European Research Council (ERC) starting grant {"}MICROROBOTS{"} (to L.G., M.M., K.S., and B.D.).*%blankline%* Publisher Copyright: {\textcopyright} 2019 National Academy of Sciences. All rights reserved.",
year = "2019",
month = dec,
day = "10",
doi = "10.1073/pnas.1911262116",
language = "English",
volume = "116",
pages = "25278--25286",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "NATL ACAD SCIENCES",
number = "50",
}