Assays to Monitor Autophagy Progression in Cell Cultures

Idil Orhon, Fulvio Reggiori

OnderzoeksoutputAcademicpeer review

41 Citaten (Scopus)
293 Downloads (Pure)

Samenvatting

The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design.

Originele taal-2English
Artikelnummer20
Aantal pagina's13
TijdschriftCells
Volume6
Nummer van het tijdschrift3
DOI's
StatusPublished - 7-jul-2017

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