Asymmetric inheritance of centrosome-associated primary cilium membrane directs ciliogenesis after cell division

Judith T M L Paridaen, Michaela Wilsch-Bräuninger, Wieland B Huttner

OnderzoeksoutputAcademicpeer review

231 Citaten (Scopus)

Samenvatting

Primary cilia are key sensory organelles that are thought to be disassembled prior to mitosis. Inheritance of the mother centriole, which nucleates the primary cilium, in relation to asymmetric daughter cell behavior has previously been studied. However, the fate of the ciliary membrane upon cell division is unknown. Here, we followed the ciliary membrane in dividing embryonic neocortical stem cells and cultured cells. Ciliary membrane attached to the mother centriole was endocytosed at mitosis onset, persisted through mitosis at one spindle pole, and was asymmetrically inherited by one daughter cell, which retained stem cell character. This daughter re-established a primary cilium harboring an activated signal transducer earlier than the noninheriting daughter. Centrosomal association of ciliary membrane in dividing neural stem cells decreased at late neurogenesis when these cells differentiate. Our data imply that centrosome-associated ciliary membrane acts as a determinant for the temporal-spatial control of ciliogenesis.

Originele taal-2English
Pagina's (van-tot)333-344
Aantal pagina's12
TijdschriftCell
Volume155
Nummer van het tijdschrift2
DOI's
StatusPublished - 10-okt.-2013
Extern gepubliceerdJa

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