Atg23 is essential for the cytoplasm to vacuole targeting pathway and efficient autophagy but not pexophagy

Katherine A Tucker, Fulvio Reggiori, William A Dunn, Daniel J Klionsky, Fulvio Reggiori

OnderzoeksoutputAcademicpeer review

74 Citaten (Scopus)

Samenvatting

Cells must regulate both biosynthesis and degradation to ensure proper homeostasis of cellular organelles and proteins. This balance is demonstrated in a unique way in the yeast Saccharomyces cerevisiae, which possesses two distinct, yet mechanistically related trafficking routes mediating the delivery of proteins from the cytoplasm to the vacuole: the biosynthetic cytoplasm to vacuole targeting (Cvt) and the degradative autophagy pathways. Several components employed by these two transport routes have been identified, but their mechanistic interactions remain largely unknown. Here we report a novel gene involved in these pathways, which we have named ATG23. Atg23 localizes to the pre-auto-phagosomal structure but also to other cytosolic punctate compartments. Our characterization of the Atg23 protein indicates that it is required for the Cvt pathway and efficient autophagy but not pexophagy. In the absence of Atg23, cargo molecules such as prApe1 are correctly recruited to a pre-autophagosomal structure that is unable to give rise to Cvt vesicles. We also demonstrate that Atg23 is a peripheral membrane protein that requires the presence of Atg9/Apg9 to be specifically targeted to lipid bilayers. Atg9 transiently interacts with Atg23 suggesting that it participates in the recruitment of this protein.

Originele taal-2English
Pagina's (van-tot)48445-52
Aantal pagina's8
TijdschriftThe Journal of Biological Chemistry
Volume278
Nummer van het tijdschrift48
DOI's
StatusPublished - 28-nov.-2003
Extern gepubliceerdJa

Citeer dit