B cell lymphoma: Characterization of the microenvironment and the tumor cell proteome

Rui Wu

    Onderzoeksoutput: Thesis fully internal (DIV)

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    B cell lymphoma is the most common hematologic malignancy. Besides alterations in the malignant lymphoma cells, also the interaction with the microenvironment has gained much interest in recent years. The aim of this thesis is to characterize the malignant cells as well as the surrounding microenvironment by proteomics and by flow analysis.
    In the first part we investigated changes in the expression levels of membrane proteins of non Hodgkin lymphoma (NHL) cell lines compared with a lymphoblastoid cell line. We found differential expression of peptidylprolyl isomerase A which can be considered as a potential target for treatment of lymphoma patients. Next, we profiled the secretome of NHL cell lines and identified potential novel disease biomarkers such as macrophage inhibition factor and CD70.
    In the second part, we analyzed the composition of tonsil and reactive lymph node (RLN) to select the best normal counterpart for the subsequent analysis of the B cell lymphoma samples. Then, we compared the composition of the microenvironment of different NHL as well as Hodgkin lymphoma (HL) with RLN. We found more cytotoxic T cells in more aggressive lymphomas, these cells are probably in an hypo-immune response status since they showed high expression of an exhaustion marker. Converting the exhaustive phenotype of the T cells might present a novel treatment strategy to induce an effective immune response in B cell lymphomas. In HL we found that the percentage of T regulatory cells is high in the tumor areas and that these cells most likely suppress the immune response.
    Originele taal-2English
    KwalificatieDoctor of Philosophy
    Toekennende instantie
    • Rijksuniversiteit Groningen
    Begeleider(s)/adviseur
    • van den Berg, Anke, Supervisor
    • Chiu, Jen-Fu, Supervisor, Externe Persoon
    • Visser, Lydia, Co-supervisor
    • Diepstra, Arjan, Co-supervisor
    Datum van toekenning23-mrt-2016
    Plaats van publicatie[Groningen]
    Uitgever
    Gedrukte ISBN's978-90-367-8739-0
    Elektronische ISBN's978-90-367-8738-3
    StatusPublished - 2016

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