Bacillus subtilis Biosensor Engineered To Assess Meat Spoilage

Alicja Daszczuk, Yonathan Dessalegne, Ismael Drenth, Elbrich Hendriks, Emeraldo Jo, Tom van Lente, Arjan Oldebesten, Jonathon Parrish, Wlada Poljakova, Annisa A. Purwanto, Renske van Raaphorst, Mirjam Boonstra, Auke van Heel, Martijn Herber, Sjoerd van der Meulen, Jeroen Siebring, Robin A. Sorg, Matthias Heinemann*, Oscar P. Kuipers, Jan-Willem Veening

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: ArticleAcademicpeer review

17 Citaten (Scopus)


Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was PsboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned PsboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector. We show that the newly identified promoter could efficiently drive fluorescent protein production in B. subtilis in response to spoiled meat and thus can be used as a biosensor to detect meat spoilage.

Originele taal-2English
Pagina's (van-tot)999-1002
Aantal pagina's4
TijdschriftACS Synthetic Biology
Nummer van het tijdschrift12
StatusPublished - 19-dec.-2014

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