Bacteria-targeted infection imaging: Towards a faster diagnosis of bacterial infection

Marjolein Heuker

doi:10.33612/diss.177500497

Bacteria-targeted infection imaging: Towards a faster diagnosis of bacterial infection

Marjolein Heuker

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Bacteriële infecties vormen een veelvoorkomende en grote bedreiging voor de gezondheid van de mens. Ze zijn verantwoordelijk voor ernstige morbiditeit en mortaliteit onder patiënten. De definitieve diagnose van infectie kan alleen worden verkregen via microbiologische kweken en moleculaire detectietechnieken, wat vaak tijdrovend is. Bovendien is het soms niet mogelijk om geschikte biopten of kweekmateriaal te verkrijgen, waardoor het stellen van de juiste diagnose zeer uitdagend kan zijn. Deze problemen kunnen mogelijk omzeild worden met behulp van bacterie-specifieke beeldvormingstechnieken. Helaas zijn de huidige beeldvormende technieken om infectieziekten te diagnosticeren niet in staat om een bacteriële infectie te onderscheiden van een steriele ontsteking. Nieuwe beeldvormende technieken zijn daarom dringend nodig voor een snelle, nauwkeurige en bij voorkeur real-time diagnose van bacteriële infecties. Dit proefschrift beschrijft de ontwikkeling van nieuwe bacterie-gerichte beeldvormingstechnieken met een focus op het gebruik van fluorescente moleculen die specifiek aan bacteriën binden. De resultaten laten zien dat fluorescente beeldvorming van bacteriële infecties geschikt is voor toepassing op geëxplanteerde materialen, tijdens artroscopie of bronchoscopie, of intra-operatief. Samenvattend kan geconcludeerd worden dat bacterie-gerichte beeldvormingstechnieken goed toepasbaar zijn voor een snellere diagnose van bacteriële infecties.

Originele taal-2	English
Kwalificatie	Doctor of Philosophy
Toekennende instantie	Rijksuniversiteit Groningen
Begeleider(s)/adviseur	van Dijl, Jan Maarten, Supervisor van Dam, Go, Supervisor van Oosten, Marleen, Co-supervisor
Datum van toekenning	8-sep.-2021
Plaats van publicatie	[Groningen]
Uitgever	University of Groningen
DOI's	https://doi.org/10.33612/diss.177500497
Status	Published - 2021

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10.33612/diss.177500497

Title and contentsFinal publisher's version, 507 KB
Chapter 1Final publisher's version, 785 KB
Chapter 2Final publisher's version, 1,87 MB
Chapter 3Final publisher's version, 2,81 MB
Chapter 4Final publisher's version, 4,43 MB
Chapter 5Final publisher's version, 1,98 MB
Chapter 6Final publisher's version, 1,62 MB
Chapter 7Final publisher's version, 3,69 MB
Chapter 8Final publisher's version, 3,15 MB
Chapter 9Final publisher's version, 6,5 MB
Chapter 10Final publisher's version, 1,15 MB
Chapter 11Final publisher's version, 1,39 MB
Chapter 12Final publisher's version, 2,52 MB
Complete thesisFinal publisher's version, 22,6 MB
PropositionsFinal publisher's version, 438 KB

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@phdthesis{d43230fe8f764febb3ab72494d8e7562,

title = "Bacteria-targeted infection imaging: Towards a faster diagnosis of bacterial infection",

abstract = "Bacterial infections occur frequently and are a major threat to human health, causing high morbidity and mortality all over the world. For adequate treatment of infections, a rapid and precise diagnosis is imperative. Presently, this is achieved via microbiological culture and molecular detection, which are often time-consuming processes. Moreover, it is not always possible to obtain appropriate material for culturing, which complicates the diagnostic process. Therefore, the PhD research described in this thesis was aimed at exploring imaging-based techniques for faster and preferably real-time diagnosis of infectious diseases, and for rapid distinction of bacterial infection from sterile inflammation. To this end, the development of new bacteria-targeted imaging modalities was reviewed with a special focus on bacteria-targeted imaging with fluorescent tracers. Subsequently, the possible use of different tracers for infection imaging was experimentally explored. The results show that imaging with fluorescent bacteria-targeted tracers can be readily applied to detect infection ex vivo, by arthroscopy or bronchoscopy, or intra-operatively. Altogether, it is concluded that bacteria-targeted fluorescence imaging approaches may allow faster, real-time diagnosis of bacterial infections.",

author = "Marjolein Heuker",

year = "2021",

doi = "10.33612/diss.177500497",

language = "English",

publisher = "University of Groningen",

school = "University of Groningen",

}

TY - BOOK

T1 - Bacteria-targeted infection imaging

T2 - Towards a faster diagnosis of bacterial infection

AU - Heuker, Marjolein

PY - 2021

Y1 - 2021

N2 - Bacterial infections occur frequently and are a major threat to human health, causing high morbidity and mortality all over the world. For adequate treatment of infections, a rapid and precise diagnosis is imperative. Presently, this is achieved via microbiological culture and molecular detection, which are often time-consuming processes. Moreover, it is not always possible to obtain appropriate material for culturing, which complicates the diagnostic process. Therefore, the PhD research described in this thesis was aimed at exploring imaging-based techniques for faster and preferably real-time diagnosis of infectious diseases, and for rapid distinction of bacterial infection from sterile inflammation. To this end, the development of new bacteria-targeted imaging modalities was reviewed with a special focus on bacteria-targeted imaging with fluorescent tracers. Subsequently, the possible use of different tracers for infection imaging was experimentally explored. The results show that imaging with fluorescent bacteria-targeted tracers can be readily applied to detect infection ex vivo, by arthroscopy or bronchoscopy, or intra-operatively. Altogether, it is concluded that bacteria-targeted fluorescence imaging approaches may allow faster, real-time diagnosis of bacterial infections.

AB - Bacterial infections occur frequently and are a major threat to human health, causing high morbidity and mortality all over the world. For adequate treatment of infections, a rapid and precise diagnosis is imperative. Presently, this is achieved via microbiological culture and molecular detection, which are often time-consuming processes. Moreover, it is not always possible to obtain appropriate material for culturing, which complicates the diagnostic process. Therefore, the PhD research described in this thesis was aimed at exploring imaging-based techniques for faster and preferably real-time diagnosis of infectious diseases, and for rapid distinction of bacterial infection from sterile inflammation. To this end, the development of new bacteria-targeted imaging modalities was reviewed with a special focus on bacteria-targeted imaging with fluorescent tracers. Subsequently, the possible use of different tracers for infection imaging was experimentally explored. The results show that imaging with fluorescent bacteria-targeted tracers can be readily applied to detect infection ex vivo, by arthroscopy or bronchoscopy, or intra-operatively. Altogether, it is concluded that bacteria-targeted fluorescence imaging approaches may allow faster, real-time diagnosis of bacterial infections.

U2 - 10.33612/diss.177500497

DO - 10.33612/diss.177500497

M3 - Thesis fully internal (DIV)

PB - University of Groningen

CY - [Groningen]

ER -

Bacteria-targeted infection imaging: Towards a faster diagnosis of bacterial infection

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