Barcoded vector libraries and retroviral or lentiviral barcoding of hematopoietic stem cells

Leonid V. Bystrykh, Gerald De Haan, Evgenia Verovskaya

OnderzoeksoutputAcademicpeer review

10 Citaten (Scopus)


Cellular barcoding is a relatively recent technique aimed at clonal analysis of a proliferating cell population of any kind. The method was shown to be particularly successful in monitoring clonal contributions of hematopoietic stem cells (HSCs). An essential step of the method is retroviral or lentiviral labeling of the hematopoietic cells. The unique feature of the method is the generation of a vector library containing specific artificial DNA tags, generally known as barcodes. The library must satisfy multiple essential requirements. Importantly, considering the number of possible variations within the barcode sequence, the actual size of the barcoded vector library, and the number of clonogenic (stem) cells in the given experiment should be in ratios far from saturation. Excessive bias in barcodes frequencies must be avoided, and the library size must be assessed prior to the sequencing analysis. The final sequencing results must undergo statistical filtering. If all requirements are met, the method ensures profound sensitivity and accuracy for monitoring of the clonal fluctuations in a wide range of biological experiments.

Originele taal-2English
TitelHematopoietic Stem Cell Protocols
UitgeverijHumana Press
Aantal pagina's16
ISBN van geprinte versie9781493911325
StatusPublished - 1-jan-2014

Publicatie series

NaamMethods in Molecular Biology
ISSN van geprinte versie1064-3745

Citeer dit