Behaviour of chromosomes in potato leaf tissue cultured in vitro as studied by BrdC-Giemsa labelling

L. P. Pijnacker*, K. Walch, M. A. Ferwerda

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    18 Citaten (Scopus)

    Samenvatting

    Cells of leaf explants of a monohaploid potato (Solanum tuberosum) were stimulated to mitosis on a medium with 5-bromodeoxycytidine during a period of 7 days. The cells cycled with mono- or diplochromosomes which showed differential staining of the sister chromatids and sister chromatid exchanges by the fluorescent plus Giemsa technique after two rounds of BrdC incorporation. Through the staining pattern the course of the first three cell cycles could be traced and the duration of the cycles estimated. Polyploidisation was enhanced by selective stimulation of polyploid cells and by endoreduplication of G2-phase cells. The percentage of polyploid mitoses increased from 10 to 70
    Originele taal-2English
    Pagina's (van-tot)833-839
    Aantal pagina's7
    TijdschriftTheoretical and Applied Genetics
    Volume72
    Nummer van het tijdschrift6
    DOI's
    StatusPublished - 1986

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