Bioconjugation Strategies for Connecting Proteins to DNA-Linkers for Single-Molecule Force-Based Experiments

Lyan M. van der Sleen, Katarzyna M. Tych*

*Bijbehorende auteur voor dit werk

Onderzoeksoutputpeer review

10 Citaten (Scopus)
53 Downloads (Pure)

Samenvatting

The mechanical properties of proteins can be studied with single molecule force spectroscopy (SMFS) using optical tweezers, atomic force microscopy and magnetic tweezers. It is common to utilize a flexible linker between the protein and trapped probe to exclude short-range interactions in SMFS experiments. One of the most prevalent linkers is DNA due to its well-defined properties, although attachment strategies between the DNA linker and protein or probe may vary. We will therefore provide a general overview of the currently existing non-covalent and covalent bioconjugation strategies to site-specifically conjugate DNA-linkers to the protein of interest. In the search for a standardized conjugation strategy, considerations include their mechanical properties in the context of SMFS, feasibility of site-directed labeling, labeling efficiency, and costs.

Originele taal-2English
Artikelnummer2424
Aantal pagina's10
TijdschriftNanomaterials
Volume11
Nummer van het tijdschrift9
DOI's
StatusPublished - sep.-2021

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