Bioinspired Silica Mineralization on Viral Templates

Christina Dickmeis, Klara Altintoprak, Patrick van Rijn, Christina Wege, Ulrich Commandeur

OnderzoeksoutputAcademic

11 Citaten (Scopus)

Samenvatting

Plant virus capsids are attractive entities for nanotechnological applications because of their variation in shape and natural assembly ability. This chapter describes the production and modification of three differently shaped plant virus capsids for silica mineralization purposes. The chosen plant viruses exhibit either an icosahedral (cowpea mosaic virus, CPMV), or a flexuous rod-like structure (potato virus X, PVX), or a rigid rod-like shape (tobacco mosaic virus, TMV), and are well-known and frequently used plant viruses for biotechnological applications. We describe the production (including genetic or chemical modification) and purification of the plant viruses or of empty virus-like particles in the case of CPMV, as well as the characterization of these harvested templates. The mineralization procedures and differences in the protocols specific to the distinct viruses are described, and the analyses of the mineralization results are explained.

Originele taal-2English
TitelVirus-Derived Nanoparticles for Advanced Technologies
SubtitelMethods and Protocols
RedacteurenChristina Wege, George P. Lomonossoff
Plaats van productieNew York
UitgeverijHumana Press
Pagina's337-362
Aantal pagina's26
ISBN van elektronische versie978-1-4939-7808-3
ISBN van geprinte versie978-1-4939-7806-9
DOI's
StatusPublished - 2018

Publicatie series

NaamMethods in Molecular Biology
ISSN van geprinte versie1064-3745

Vingerafdruk

Duik in de onderzoeksthema's van 'Bioinspired Silica Mineralization on Viral Templates'. Samen vormen ze een unieke vingerafdruk.

Citeer dit