Bioorthogonal metabolic labelling with acyl-CoA reporters: targeting protein acylation

Maria E. Ourailidou, Martijn R.H. Zwinderman, Frans Dekker

Onderzoeksoutputpeer review

6 Citaten (Scopus)
22 Downloads (Pure)

Samenvatting

Protein acylation is an abundant post-translational modification with a pivotal role in a plethora of biological processes. To date, metabolic labelling with functionalized precursors of acyl-CoA and subsequent bio-orthogonal ligation to a complementary detection tag has offered an attractive approach for monitoring endogenous protein acylation with excellent selectivity. This review focuses on the applications of alkyne- and alkene-based bioorthogonal chemistries in the study of enzyme activity in vitro and summarizes the carboxylate-type chemical reporters that have enabled the visualization and identification of cellular acylated proteins. However, despite their importance, serious limitations question the use of this two-step labelling method in the quantification of the protein acylome.

Originele taal-2English
Pagina's (van-tot)399-408
Aantal pagina's10
TijdschriftMedChemCommun
Volume7
Nummer van het tijdschrift3
DOI's
StatusPublished - 2016

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