Blending Baeyer-Villiger monooxygenases: using a robust BVMO as a scaffold for creating chimeric enzymes with novel catalytic properties

Hugo L. van Beek, Gonzalo de Gonzalo, Marco W. Fraaije*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

59 Citaten (Scopus)

Samenvatting

The thermostable Baeyer-Villiger monooxygenase (BVMO) phenylacetone monooxygenase (PAMO) is used as a scaffold to introduce novel selectivities from other BVMOs or the metagenome by structure-inspired subdomain exchanges. This yields biocatalysts with new preferences in the oxidation of sulfides and the Baeyer-Villiger oxidation of ketones, all while maintaining most of the original thermostability.

Originele taal-2English
Pagina's (van-tot)3288-3290
Aantal pagina's3
TijdschriftChemical Communications
Volume48
Nummer van het tijdschrift27
DOI's
StatusPublished - 2012

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