Candida biofilm analysis in the artificial throat using fisH

Bastiaan P. Krom, Kevin Buijssen, Henk J. Busscher, Henny C. Van Der Mei

    OnderzoeksoutputAcademicpeer review

    3 Citaten (Scopus)

    Samenvatting

    Biofilm formation is a common complication of the use of prosthetic devices. In clinical settings, biofilms can be comprised of one or more microbial species. In order to investigate the interaction between different species within a biofilm, a reproducible, reliable model system has to be utilized and an appropriate system for species identification applied. The present chapter describes the artificial throat model, a model system for growing mixed species biofilms on shunt prostheses. The model is used in conjugation with fluorescent in situ hybridization (FISH), which facilitates identification and localization of the resident microorganisms within biofilms.

    Originele taal-2English
    TitelCandida albicans
    SubtitelMethods and Protocols
    RedacteurenRonald Cihlar, Richard Calderone
    Pagina's45-54
    Aantal pagina's10
    DOI's
    StatusPublished - 2009

    Publicatie series

    NaamMethods in Molecular Biology
    Volume499
    ISSN van geprinte versie1064-3745

    Vingerafdruk

    Duik in de onderzoeksthema's van 'Candida biofilm analysis in the artificial throat using fisH'. Samen vormen ze een unieke vingerafdruk.

    Citeer dit