Cation permeability in CorA family of proteins

Artem Stetsenko, Albert Guskov*

*Corresponding author voor dit werk

Onderzoeksoutput: ArticleAcademicpeer review

10 Citaten (Scopus)
181 Downloads (Pure)

Samenvatting

CorA proteins belong to 2-TM-GxN family of membrane proteins, and play a major role in Mg2+ transport in prokaryotes and eukaryotic mitochondria. The selection of substrate is believed to occur via the signature motif GxN, however there is no consensus how strict this selection within the family. To answer this question, we employed fluorescence-based transport assays on three different family members, namely CorA from bacterium Thermotoga maritima, CorA from the archeon Methanocaldococcus jannaschii and ZntB from bacterium Escherichia coli, reconstituted into proteoliposomes. Our results show that all three proteins readily transport Mg2+, Co2+, Ni2+ and Zn2+, but not Al3+. Despite the similarity in cation specificity, ZntB differs from the CorA proteins, as in the former transport is stimulated by a proton gradient, but in the latter by the membrane potential,
confirming the hypothesis that CorA and ZntB proteins diverged to different transport mechanisms within the same protein scaffold.
Originele taal-2English
Artikelnummer840
Aantal pagina's9
TijdschriftScientific Reports
Volume10
Nummer van het tijdschrift1
DOI's
StatusPublished - 1-dec.-2020

Vingerafdruk

Duik in de onderzoeksthema's van 'Cation permeability in CorA family of proteins'. Samen vormen ze een unieke vingerafdruk.

Citeer dit