Cationic amphiphiles as delivery system for genes into eukaryotic cells

Volker Oberle, Inge S. Zuhorn, Sandrine Audouy, Udo Bakowsky, Jarmila Smisterová, Jan B.F.N. Engberts, Dick Hoekstra

OnderzoeksoutputAcademicpeer review

88 Downloads (Pure)

Samenvatting

Cationic liposomes, consisting of synthetic amphiphiles and a so-called helper lipid, rapidly form complexes with DNA, known as lipoplexes. When incubated with cells in culture, the DNA can be delivered into the cell and becomes expressed. Because of these properties, lipoplexes are considered a useful alternative for viral vectors for in vivo genetherapy. Yet, many hurdles have still to be taken. These an illustrated in the present overview, which briefly describes the critical steps involved in overall gene delivery in vitro, ranging from lipoplex formation to nuclear penetration and delivery of the desired gene.

Originele taal-2English
TitelTARGETING OF DRUGS
RedacteurenG Gregoriadis, B McCormack
Plaats van productieAMSTERDAM
UitgeverijI O S PRESS
Pagina's146-155
Aantal pagina's10
ISBN van geprinte versie1-58603-009-4
StatusPublished - 2000
Evenement10th Meeting of the NATO-Advanced-Studies-Institute on Targeting of Drugs: Strategies for Gene Constructs and Delivery - , Greece
Duur: 24-jun-19995-jul-1999

Publicatie series

NaamNATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES
UitgeverijI O S PRESS
Volume323
ISSN van geprinte versie0258-1213

Other

Other10th Meeting of the NATO-Advanced-Studies-Institute on Targeting of Drugs: Strategies for Gene Constructs and Delivery
Land/RegioGreece
Periode24/06/199905/07/1999

Citeer dit