CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

SC MICHAELIS; W BARDENHEUER; A LUX; A SCHRAMM; A GOCKEL; R SIEBERT; C WILLERS; K SCHMIDTKE; B TODT; AH VANDERHOUT; CHCM BUYS; AC HEPPELLPARTON; PH RABBITTS; S UNGAR; D SMITH; D LEPASLIER; D COHEN; B OPALKA; J SCHUTTE

CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

SC MICHAELIS^*
, W BARDENHEUER
, A LUX
, A SCHRAMM
, A GOCKEL
, R SIEBERT
, C WILLERS
, K SCHMIDTKE
, B TODT
, AH VANDERHOUT
, CHCM BUYS
, AC HEPPELLPARTON
, PH RABBITTS
, S UNGAR
, D SMITH
, D LEPASLIER
, D COHEN
, B OPALKA
, J SCHUTTE

^*Corresponding author voor dit werk

Onderzoeksoutput: Article › Academic › peer review

18 Citaten (Scopus)

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Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH wets in agreement with the STS-based arrangement of the YAC-contig.

Originele taal-2	English
Pagina's (van-tot)	1-12
Aantal pagina's	12
Tijdschrift	Cancer Genetics and Cytogenetics
Volume	81
Nummer van het tijdschrift	1
Status	Published - mei-1995

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MICHAELIS, SC., BARDENHEUER, W., LUX, A., SCHRAMM, A., GOCKEL, A., SIEBERT, R., WILLERS, C., SCHMIDTKE, K., TODT, B., VANDERHOUT, AH., BUYS, CHCM., HEPPELLPARTON, AC., RABBITTS, PH., UNGAR, S., SMITH, D., LEPASLIER, D., COHEN, D., OPALKA, B., & SCHUTTE, J. (1995). CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES. Cancer Genetics and Cytogenetics, 81(1), 1-12.

@article{4c73339720f24066bcbb1928689b4afb,

title = "CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES",

abstract = "Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH wets in agreement with the STS-based arrangement of the YAC-contig.",

keywords = "RENAL-CELL CARCINOMA, DELETION MAPPING PANEL, LUNG-CANCER, SHORT ARM, INSITU HYBRIDIZATION, GENETIC ALTERATIONS, ALLELIC LOSS, HUMAN GENOME, TRANSLOCATION, LOCALIZATION",

author = "SC MICHAELIS and W BARDENHEUER and A LUX and A SCHRAMM and A GOCKEL and R SIEBERT and C WILLERS and K SCHMIDTKE and B TODT and AH VANDERHOUT and CHCM BUYS and AC HEPPELLPARTON and PH RABBITTS and S UNGAR and D SMITH and D LEPASLIER and D COHEN and B OPALKA and J SCHUTTE",

year = "1995",

month = may,

language = "English",

volume = "81",

pages = "1--12",

journal = "Cancer Genetics and Cytogenetics",

issn = "0165-4608",

publisher = "ELSEVIER SCIENCE INC",

number = "1",

}

MICHAELIS, SC, BARDENHEUER, W, LUX, A, SCHRAMM, A, GOCKEL, A, SIEBERT, R, WILLERS, C, SCHMIDTKE, K, TODT, B, VANDERHOUT, AH, BUYS, CHCM, HEPPELLPARTON, AC, RABBITTS, PH, UNGAR, S, SMITH, D, LEPASLIER, D, COHEN, D, OPALKA, B & SCHUTTE, J 1995, 'CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES', Cancer Genetics and Cytogenetics, vol. 81, nr. 1, blz. 1-12.

TY - JOUR

T1 - CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

AU - MICHAELIS, SC

AU - BARDENHEUER, W

AU - LUX, A

AU - SCHRAMM, A

AU - GOCKEL, A

AU - SIEBERT, R

AU - WILLERS, C

AU - SCHMIDTKE, K

AU - TODT, B

AU - VANDERHOUT, AH

AU - BUYS, CHCM

AU - HEPPELLPARTON, AC

AU - RABBITTS, PH

AU - UNGAR, S

AU - SMITH, D

AU - LEPASLIER, D

AU - COHEN, D

AU - OPALKA, B

AU - SCHUTTE, J

PY - 1995/5

Y1 - 1995/5

N2 - Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH wets in agreement with the STS-based arrangement of the YAC-contig.

AB - Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH wets in agreement with the STS-based arrangement of the YAC-contig.

KW - RENAL-CELL CARCINOMA

KW - DELETION MAPPING PANEL

KW - LUNG-CANCER

KW - SHORT ARM

KW - INSITU HYBRIDIZATION

KW - GENETIC ALTERATIONS

KW - ALLELIC LOSS

KW - HUMAN GENOME

KW - TRANSLOCATION

KW - LOCALIZATION

M3 - Article

SN - 0165-4608

VL - 81

SP - 1

EP - 12

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

IS - 1

ER -

CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

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