Characterization of antigen-presenting properties of tumour cells using virus-specific cytotoxic T lymphocytes

DCJ Spierings, E Agsteribbe, J Wilschut, A Huckriede*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

5 Citaten (Scopus)
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Samenvatting

Immunotherapy of tumours by induction of tumour-specific cytotoxic T-lymphocytes (CTLs) will only be effective for tumours with a functional antigen processing and presentation machinery. However, many tumours are known to down-regulate expression of major histocompatibility complex (MHC) class I molecules and/or to impair antigen processing. It is therefore desirable to evaluate the ability of a given tumour to present antigenic epitopes before developing an immunotherapy protocol. In this study we have used influenza virus as a tool to determine the antigen-presenting capacities of the murine neuroblastoma C1300 cell line NB41A3, a frequently used model far human neuroblastoma. Immunofluorescence analyses revealed low and moderate expression of MHC class I molecules D-d and K-k respectively. Nevertheless, infected NB41A3 cells were lysed efficiently by influenza-specific CTLs. These results demonstrate that all steps of the antigen-processing pathway function properly in the NE tumour cells, and that the limited MHC class I expression suffices far efficient recognition by CTLs. In addition, lysis of the NE tumour cells shows that the cells are susceptible to CTL-induced apoptosis, a pathway that is often impaired in tumour cells. These characteristics make neuroblastoma a suitable target for immunotherapy. The presented assay allows evaluation of various immunological properties of tumour cells and, thus, represents a valuable tool to assess whether a given tumour will be susceptible to Immunotherapy or not. (C) 2000 Cancer Research campaign.

Originele taal-2English
Pagina's (van-tot)1474-1479
Aantal pagina's6
TijdschriftBritish Jounal of Cancer
Volume82
Nummer van het tijdschrift8
DOI's
StatusPublished - apr-2000

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