Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa: Aves)

Yvonne I. Verkuil; Krijn Trimbos; Oliver Haddrath; Allan J. Baker; Theunis Piersma

doi:10.1111/j.1755-0998.2009.02685.x

Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa: Aves)

Yvonne I. Verkuil^*, Krijn Trimbos, Oliver Haddrath, Allan J. Baker, Theunis Piersma

^*Corresponding author voor dit werk

Piersma group - Global Flyway Ecology

Onderzoeksoutput › Academic › peer review

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We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica).

Originele taal-2	English
Pagina's (van-tot)	1415-1418
Aantal pagina's	4
Tijdschrift	Molecular Ecology Resources
Volume	9
Nummer van het tijdschrift	5
DOI's	https://doi.org/10.1111/j.1755-0998.2009.02685.x
Status	Published - sep.-2009

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title = "Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa: Aves)",

abstract = "We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica).",

keywords = "black-tailed godwit, ground-nesting shorebird, Limosa limosa, microsatellite, population genetics, SOFTWARE",

author = "Verkuil, {Yvonne I.} and Krijn Trimbos and Oliver Haddrath and Baker, {Allan J.} and Theunis Piersma",

year = "2009",

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doi = "10.1111/j.1755-0998.2009.02685.x",

language = "English",

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journal = "Molecular Ecology Resources",

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TY - JOUR

T1 - Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa

T2 - Aves)

AU - Verkuil, Yvonne I.

AU - Trimbos, Krijn

AU - Haddrath, Oliver

AU - Baker, Allan J.

AU - Piersma, Theunis

PY - 2009/9

Y1 - 2009/9

N2 - We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica).

AB - We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica).

KW - black-tailed godwit

KW - ground-nesting shorebird

KW - Limosa limosa

KW - microsatellite

KW - population genetics

KW - SOFTWARE

U2 - 10.1111/j.1755-0998.2009.02685.x

DO - 10.1111/j.1755-0998.2009.02685.x

M3 - Article

SN - 1755-098X

VL - 9

SP - 1415

EP - 1418

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 5

ER -

Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa: Aves)

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