TY - JOUR
T1 - Chemical characterisation of three haemolytic compounds from the microalgal species Fibrocapsa japonica (Raphidophyceae)
AU - Fu, M.
AU - Koulman, A
AU - van Rijssel, M
AU - Lutzen, A.
AU - de Boer, M.K.
AU - Tyl, M.R.
AU - Liebezeit, G.
N1 - Times Cited: 2 rticle nglish u, M es Ctr Terramare, Schleusenstr 1, D-26382 Wilhelmshaven, Germany ited References Count: 29 15CW HE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND XFORD
PY - 2004
Y1 - 2004
N2 - The molecular Structures of the three main haemolytic compounds (Fj1, Fj2 and Fj3) isolated from the ichthyotoxic microalgal species Fibrocapsa japonica have been investigated by NMR, LC-ESI-MS, ESI-MS-MS, IR, GC-MS and GC-HRMS methods. They are polyunsaturated fatty acids which we identified as: 6,9,12,15-octadecatetraenoic acid (OTA, C18:4omega3). 5,8.11,14,1 7-eicosapentaenoic acid (EPA, C20:5omega3) and 5,8,11,14-eicosatetraenoic acid (arachidonic acid AA, C20:4omega6). The identity of the latter two was confirmed on the basis of commercial standards (C20:5omega3 and C20:4omega6). Both displayed the same spectroscopic and chromatographic characteristics as Fj2 and Fj3 and had a similar strong haemolytic effect. We propose that when F. japonica cells accumulate in fish gills during blooms these compounds could be the cause of icthyotoxicity. (C) 2003 Published by Elsevier Ltd
AB - The molecular Structures of the three main haemolytic compounds (Fj1, Fj2 and Fj3) isolated from the ichthyotoxic microalgal species Fibrocapsa japonica have been investigated by NMR, LC-ESI-MS, ESI-MS-MS, IR, GC-MS and GC-HRMS methods. They are polyunsaturated fatty acids which we identified as: 6,9,12,15-octadecatetraenoic acid (OTA, C18:4omega3). 5,8.11,14,1 7-eicosapentaenoic acid (EPA, C20:5omega3) and 5,8,11,14-eicosatetraenoic acid (arachidonic acid AA, C20:4omega6). The identity of the latter two was confirmed on the basis of commercial standards (C20:5omega3 and C20:4omega6). Both displayed the same spectroscopic and chromatographic characteristics as Fj2 and Fj3 and had a similar strong haemolytic effect. We propose that when F. japonica cells accumulate in fish gills during blooms these compounds could be the cause of icthyotoxicity. (C) 2003 Published by Elsevier Ltd
KW - Fibrocapsa japonica
KW - haemolytic compounds
KW - 6,9,12,15-octadecatetraenoic acid (OTA,C18 : 4 omega 3)
KW - 5,8,11,14,17-eicosapentaenoic acid (EPA, C20 : 5 omega 3)
KW - 5,8,11,14-eicosatetraenoic acid (arachidonic acid AA, C20 : 4 omega 6)
KW - high performance liquid chromatography
KW - nuclear magnetic resonance
KW - infrared spectroscopy
KW - liquid chromatography-electrospray ionisation-mass spectrometry
KW - gas chromatography-mass spectrometry
KW - gas chromatography-high resolution mass spectrometry
KW - erythrocyte lysis assay
KW - POLYUNSATURATED FATTY-ACIDS
KW - PHYTOPLANKTON
KW - GENERATION
KW - ORGANISMS
KW - ALGAE
U2 - 10.1016/j.toxicon.2003.09.012
DO - 10.1016/j.toxicon.2003.09.012
M3 - Article
SN - 0041-0101
VL - 43
SP - 355
EP - 363
JO - Toxicon
JF - Toxicon
IS - 4
ER -