Chimeric RNA ASTN2-PAPPA(as) aggravates tumor progression and metastasis in human esophageal cancer

Lu Wang, Xiao Xiong, Zhimeng Yao, Jianlin Zhu, Yusheng Lin, Wan Lin, Kai Li, Xiaozheng Xu, Yi Guo, Yuping Chen, Yunlong Pan, Fuyou Zhou, Jun Fan, Yan Chen, Shegan Gao*, Sai-Ching Jim Yeung, Hao Zhang

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    1 Citaat (Scopus)
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    Samenvatting

    Transcription-induced chimeric RNAs are an emerging area of research into molecular signatures for disease biomarker and therapeutic target development. Despite their importance, little is known for chimeric RNAs-relevant roles and the underlying mechanisms for cancer pathogenesis and progression. Here we describe a unique ASTN2-PAPPA(antisense) chimeric RNA (A-P-as chiRNA) that could be the first reported chimeric RNA derived from the splicing of exons and intron antisense of two neighboring genes, respectively. Aberrant A-P-as chiRNA level in ESCC tissues was associated with tumor progression and patients' outcome. In vitro and in vivo studies demonstrated that A-P-as chiRNA aggravated ESCC metastasis and enhanced stemness through modulating OCT4. Mechanistic studies demonstrated that ERK5-mediated non-canonical PAF1 activity was required for A-P-as chiRNA-induced cancer malignancy. The study defined an undocumented function of chimeric RNAs in aggravating cancer stemness and metastasis.

    Originele taal-2English
    Pagina's (van-tot)1-11
    Aantal pagina's11
    TijdschriftCancer letters
    Volume501
    DOI's
    StatusPublished - 31-mrt-2021

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