Clearing-induced tissue shrinkage: A novel observation of a thickness size effect

R. C. M. Vulders, R. C. van Hoogenhuizen, E. van der Giessen, Pieter van der Zaag*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

2 Citaten (Scopus)
27 Downloads (Pure)


The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.
Originele taal-2English
Aantal pagina's10
TijdschriftPLoS ONE
Nummer van het tijdschrift12
StatusPublished - 16-dec-2021

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