Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues

Susanna Skalicky, Peter J. Zwiers, Timara Kuiper, Elisabeth Schraml, Matthias Hackl*, Grietje Molema

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: ArticleAcademicpeer review

6 Citaten (Scopus)
114 Downloads (Pure)


Neglecting tissue heterogeneity during the analysis of microRNA (miRNA) levels results in average signals from an unknown mixture of different cell types that are difficult to interpret. Here we demonstrate the technical requirements needed to obtain high-quality, quantitative miRNA expression infor- mation from tumor tissue compartments obtained by laser microdissection (LMD). Furthermore, we show the significance of disentangling tumor tissue heterogeneity by applying the newly developed protocols for combining LMD of tumor tissue compartments with RT-qPCR analysis to reveal compartment- specific miRNA expression signatures. An important advantage of this strategy is that the miRNA signature can be directly linked to histopatho logy. In summary, combining LMD and RT-qPCR is a powerful approach for spatial miRNA expression analysis in complex tissues, enabling discovery of disease mechanisms, biomarkers and drug candidates.

Originele taal-2English
Pagina's (van-tot)276-285
Aantal pagina's10
Nummer van het tijdschrift6
StatusPublished - dec.-2019

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