Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study

Joëlle C Schutten; Adrian Post; Margriet van der Meer; Jan IJmker; Frans Goorman; Richard M Danel; Marc G Vervloet; Martin H de Borst; Daan J Touw; Stephan J L Bakker

doi:10.1016/j.cca.2020.09.007

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study

Joëlle C Schutten^*, Adrian Post, Margriet van der Meer, Jan IJmker, Frans Goorman, Richard M Danel, Marc G Vervloet, Martin H de Borst, Daan J Touw, Stephan J L Bakker

^*Bijbehorende auteur voor dit werk

Onderzoeksoutput › Academic › peer review

28 Downloads (Pure)

Samenvatting

Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.

Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.

Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.

Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

Originele taal-2	English
Pagina's (van-tot)	772-780
Aantal pagina's	9
Tijdschrift	Clinica chimica acta
Volume	510
Vroegere onlinedatum	10-sep-2020
DOI's	https://doi.org/10.1016/j.cca.2020.09.007
Status	Published - nov-2020

Toegang tot document

10.1016/j.cca.2020.09.007Licentie: CC BY

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinantsFinal publisher's version, 1,48 MBLicentie: CC BY

Lifelines Biobank
Bakker, S. (Creator), Dotinga, A. (Creator), Vonk, J. (Creator), Smidt, N. (Creator), Scholtens, S. (Creator), Swertz, M. (Creator), Wijmenga, C. (Creator), Wolffenbuttel, B. (Creator), Stolk, R. (Creator), van Zon, S. (Creator), Rosmalen, J. (Creator), Postma, D. S. (Creator), de Boer, R. (Creator), Navis, G. (Creator), Slaets, J. (Creator), Ormel, H. (Creator), van Dijk, F. (Creator) & Bolmer, B. (Data Manager), Lifelines, 2006
https://www.lifelines.nl/ en nog één link, https://catalogue.lifelines.nl/ (minder tonen)
Dataset

Citeer dit

Schutten, J. C., Post, A., van der Meer, M., IJmker, J., Goorman, F., Danel, R. M., Vervloet, M. G., de Borst, M. H., Touw, D. J., & Bakker, S. J. L. (2020). Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study. Clinica chimica acta, 510, 772-780. https://doi.org/10.1016/j.cca.2020.09.007

Schutten, Joëlle C ; Post, Adrian ; van der Meer, Margriet ; IJmker, Jan ; Goorman, Frans ; Danel, Richard M ; Vervloet, Marc G ; de Borst, Martin H ; Touw, Daan J ; Bakker, Stephan J L. / Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants : Results from the LifeLines cohort study. In: Clinica chimica acta. 2020 ; Vol. 510. blz. 772-780.

@article{67897c92c6c5441fa839d1eb61a60c96,

title = "Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study",

abstract = "Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.",

keywords = "Intra-erythrocyte magnesium, Metabolism, Method comparison, Inductively coupled plasma mass spectrometry, SERUM MAGNESIUM, CELL MAGNESIUM, RISK, URINARY, PLASMA, HYPOMAGNESEMIA, HYPERTENSION, UPDATE",

author = "Schutten, {Jo{\"e}lle C} and Adrian Post and {van der Meer}, Margriet and Jan IJmker and Frans Goorman and Danel, {Richard M} and Vervloet, {Marc G} and {de Borst}, {Martin H} and Touw, {Daan J} and Bakker, {Stephan J L}",

year = "2020",

month = nov,

doi = "10.1016/j.cca.2020.09.007",

language = "English",

volume = "510",

pages = "772--780",

journal = "Clinica chimica acta",

issn = "0009-8981",

publisher = "ELSEVIER SCIENCE BV",

}

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants : Results from the LifeLines cohort study. / Schutten, Joëlle C ; Post, Adrian; van der Meer, Margriet; IJmker, Jan; Goorman, Frans; Danel, Richard M; Vervloet, Marc G; de Borst, Martin H ; Touw, Daan J ; Bakker, Stephan J L.

In: Clinica chimica acta, Vol. 510, 11.2020, blz. 772-780.

Onderzoeksoutput › Academic › peer review

TY - JOUR

T1 - Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants

T2 - Results from the LifeLines cohort study

AU - Schutten, Joëlle C

AU - Post, Adrian

AU - van der Meer, Margriet

AU - IJmker, Jan

AU - Goorman, Frans

AU - Danel, Richard M

AU - Vervloet, Marc G

AU - de Borst, Martin H

AU - Touw, Daan J

AU - Bakker, Stephan J L

PY - 2020/11

Y1 - 2020/11

N2 - Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

AB - Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

KW - Intra-erythrocyte magnesium

KW - Metabolism

KW - Method comparison

KW - Inductively coupled plasma mass spectrometry

KW - SERUM MAGNESIUM

KW - CELL MAGNESIUM

KW - RISK

KW - URINARY

KW - PLASMA

KW - HYPOMAGNESEMIA

KW - HYPERTENSION

KW - UPDATE

U2 - 10.1016/j.cca.2020.09.007

DO - 10.1016/j.cca.2020.09.007

M3 - Article

C2 - 32919943

VL - 510

SP - 772

EP - 780

JO - Clinica chimica acta

JF - Clinica chimica acta

SN - 0009-8981

ER -

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study

Samenvatting

Toegang tot document

Datasets

Lifelines Biobank

Citeer dit