Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study

Joëlle C Schutten; Adrian Post; Margriet van der Meer; Jan IJmker; Frans Goorman; Richard M Danel; Marc G Vervloet; Martin H de Borst; Daan J Touw; Stephan J L Bakker

doi:10.1016/j.cca.2020.09.007

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study

Joëlle C Schutten^*, Adrian Post, Margriet van der Meer, Jan IJmker, Frans Goorman, Richard M Danel, Marc G Vervloet, Martin H de Borst, Daan J Touw, Stephan J L Bakker

^*Bijbehorende auteur voor dit werk

Onderzoeksoutput › Academic › peer review

2 Citaten (Scopus)

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Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.

Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.

Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.

Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

Originele taal-2	English
Pagina's (van-tot)	772-780
Aantal pagina's	9
Tijdschrift	Clinica chimica acta
Volume	510
Vroegere onlinedatum	10-sep.-2020
DOI's	https://doi.org/10.1016/j.cca.2020.09.007
Status	Published - nov.-2020

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Schutten, J. C., Post, A., van der Meer, M., IJmker, J., Goorman, F., Danel, R. M., Vervloet, M. G., de Borst, M. H., Touw, D. J., & Bakker, S. J. L. (2020). Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study. Clinica chimica acta, 510, 772-780. https://doi.org/10.1016/j.cca.2020.09.007

@article{67897c92c6c5441fa839d1eb61a60c96,

title = "Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study",

abstract = "Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.",

keywords = "Intra-erythrocyte magnesium, Metabolism, Method comparison, Inductively coupled plasma mass spectrometry, SERUM MAGNESIUM, CELL MAGNESIUM, RISK, URINARY, PLASMA, HYPOMAGNESEMIA, HYPERTENSION, UPDATE",

author = "Schutten, {Jo{\"e}lle C} and Adrian Post and {van der Meer}, Margriet and Jan IJmker and Frans Goorman and Danel, {Richard M} and Vervloet, {Marc G} and {de Borst}, {Martin H} and Touw, {Daan J} and Bakker, {Stephan J L}",

year = "2020",

month = nov,

doi = "10.1016/j.cca.2020.09.007",

language = "English",

volume = "510",

pages = "772--780",

journal = "Clinica chimica acta",

issn = "0009-8981",

publisher = "ELSEVIER SCIENCE BV",

}

TY - JOUR

T1 - Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants

T2 - Results from the LifeLines cohort study

AU - Schutten, Joëlle C

AU - Post, Adrian

AU - van der Meer, Margriet

AU - IJmker, Jan

AU - Goorman, Frans

AU - Danel, Richard M

AU - Vervloet, Marc G

AU - de Borst, Martin H

AU - Touw, Daan J

AU - Bakker, Stephan J L

PY - 2020/11

Y1 - 2020/11

N2 - Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

AB - Background: Direct methods for the assessment of infra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of infra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.Results: Mean dIEM and iIEM were 0.20 +/- 0.04 mmol/10(12) cells and 0.25 +/- 0.04 mmol/10(12) cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

KW - Intra-erythrocyte magnesium

KW - Metabolism

KW - Method comparison

KW - Inductively coupled plasma mass spectrometry

KW - SERUM MAGNESIUM

KW - CELL MAGNESIUM

KW - RISK

KW - URINARY

KW - PLASMA

KW - HYPOMAGNESEMIA

KW - HYPERTENSION

KW - UPDATE

U2 - 10.1016/j.cca.2020.09.007

DO - 10.1016/j.cca.2020.09.007

M3 - Article

C2 - 32919943

VL - 510

SP - 772

EP - 780

JO - Clinica chimica acta

JF - Clinica chimica acta

SN - 0009-8981

ER -

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study

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